Artigos em revistas internacionais_ESAS
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- Molecular and hydraulic responses of grapevine to water status and phenology under long-term differential irrigation treatmentsPublication . Damásio, M.; Pinto, C.; Salguero, J.; Alarcón, M. V.; Deus, J.; David, T. S.; Silvestre, J.; Carvalho, L. C.; Zarrouk, OlfaUnderstanding seasonal interactions among hydraulic, chemical and molecular signalling under water deficit is crucial for improving vineyard irrigation strategies under climate change and increasing water scarcity. We aimed to test how irrigation strategies and phenology affect the hydraulic adjustment mechanisms of eight fieldgrown grapevine varieties under different long-term irrigation regimes: Full-irrigated (100 % ETc), deficitirrigation (50 % Full-irrigated), and non-irrigated. Leaf pre-dawn water potential, leaf hydraulic conductivity (Kleaf), stomatal conductance, aquaporin gene expression and hormone content, were assessed at four key phenological stages, from pea-size to post-harvest. Stomatal closure was the earliest water-stress response across genotypes, while hormonal balance differentiated responsive from non-responsive genotypes regarding chemical signalling. Kleaf was primarily driven by phenology, peaking at early stages and declining thereafter. Aquaporin gene expression was both genotype- and phenology-dependent, with high activity during early stages followed by progressive down-regulation, aligned with changes in stomatal conductance and Kleaf and reflecting each variety iso-/anisohydric behaviour. This pattern confirms the role of aquaporins in grapevine hydraulic adjustments and stomatal regulation under decreasing water potentials. Aquaporins and Kleaf showed positive responses to water availability at post-harvest, indicating differential watering benefits among genotypes. Our results emphasize the need for field-phenotyping studies to fine-tune water management strategies in viticulture.
- Current context of Cannabis sativa cultivation and parameters influencing its developmentPublication . Saragoça, Andreia; Silva, Ana; Varanda, Carla; Materatski, Patrick; Ortega, Alfonso; Cordeiro, Ana; Gama, JoséCannabis sativa L. is a versatile plant with significant medicinal, industrial, and recreational applications. Its therapeutic potential is attributed to cannabinoids like THC and CBD, whose production is influenced by environmental factors, such as radiation, temperature, and humidity. Radiation, for instance, is essential for photosynthetic processes, acting as both a primary energy source and a regulator of plant growth and development. This review covers key factors affecting C. sativa cultivation, including photoperiod, light spectrum, cultivation methods, environmental controls, and plant growth regulators. It highlights how these elements influence flowering, biomass, and cannabinoid production across different growing systems, offering insights for optimizing both medicinal and industrial cannabis cultivation. Studies indicate that photoperiod sensitivity varies among cultivars, with some achieving optimal flowering and cannabinoid production under extended light periods rather than the traditional 12/12 h cycle. Light spectrum adjustments, especially red, far-red, and blue wavelengths, significantly impact photosynthesis, plant morphology, and secondary metabolite accumulation. Advances in LED technology allow precise spectral control, enhancing energy efficiency and cannabinoid profiles compared to conventional lighting. The photoperiod plays a vital role in the cultivation of C. sativa spp., directly impacting the plant’s developmental cycle, biomass production, and the concentration of cannabinoids and terpenes. The response to photoperiod varies among different cannabis cultivars, as demonstrated in studies comparing cultivars of diverse genetic origins. On the other hand, indoor or in vitro cultivation may serve as an excellent alternative for plant breeding programs in C. sativa, given the substantial inter-cultivar variability that hinders the fixation of desirable traits.
- Plant diseases and sustainable agriculturePublication . Materatski, Patrick; Vicente, Cláudia; Varanda, Carla; Espada, Margarida
- Reproductive traits of the European catfish, Silurus glanis, during the early stages of invasionPublication . Gkenas, C.; Sequeira, V.; Ribeiro, D.; Gago, João; Dias, D.; Verma, C.R.; Kumkar, P.; Ribeiro, F.In recent decades, the European catfish Silurus glanis has spread across Europe and elsewhere, significantly impacting the native fauna of the recipient ecosystems. Studies on its reproductive traits from its non-native range are limited, although this is a critical step in developing effective management plans. Here, we explore the reproductive characteristics of invasive European catfish populations in the Lower River Tagus in Portugal, focusing on its sex ratio, size at maturity, spawning period, fecundity and oocyte diameter. European catfish were collected monthly by electrofishing, gill nets, baited hooks, and professional fishermen from January 2022 to November 2023. A total of 217 females, 156 males and 301 immature fish were analysed. The female-to-male sex ratio was 1.4 : 1, with females and males reaching size at first maturity (TL50) at 72.9 and 68.8 cm total length, respectively. The spawning season was protracted, from February to June, showing asynchronous oocyte development patterns. The absolute fecundity was positively correlated with total length and total weight and ranged from 8,364 to 319,000 oocytes. Relative fecundity varied from 4.39 to 23.53 oocytes, with the mean diameter of the matured oocytes ranging from 1.50 to 3.21 mm. The results obtained in this study enhance our understanding of the reproductive biology of invasive species and can contribute to the development of effective management strategies, such as population control and density- dependent fecundity strategies.
- Nutritional and microbial quality of edible insect powder from plant-based industrial by-product and fish biowaste dietsPublication . Andrade, R.; Martins, L.L.; Mourato, M.P.; Lourenço, H.; Ramos, A.C.; Roseiro, C.; Pereira, N.; Costa, G.J.; Lucas, R.; Alvarenga, N.; Reis, J.; Neves, Ana; Oliveira, Margarida; Dias, Igor; Abreu, M.Edible insect powder, particularly from the cricket Acheta domesticus L., is a promising sustainable alternative to traditional livestock-derived protein. Insects provide high protein content, fibre, and essential minerals, making them suitable for food applications. This study investigates the viability of alternative diets for rearing A. domesticus. Two experimental diets were tested: RI [50% horticultural by-products (HP) + 50% commercial diet (CD)] and RII (33% HP + 33% CD + 33% fish by-products). The results demonstrated that both diets were suitable for cricket rearing. Crickets reared on diets RI and RII produced, respectively, insect powders FI and FII, which were evaluated for their nutritional, bioactive, and microbiological attributes. Both powders exhibited high protein content (≈60%), all essential amino acids, higher mineral content than traditional protein sources, and met European Union food safety standards. Diet composition influenced powder characteristics: FI showed higher antioxidant activity and saturated fat content, while FII contained more protein, ash, minerals, and monounsaturated fatty acids. These findings underscore the potential of using industrial by-products to promote a circulareconomy in insect farming and suggest pathways for further research. However, since insects can bioaccumulate toxic elements, such as Hg, from diets, caution should be taken when considering fish by-products
- Methods of protein extraction from house crickets (Acheta domesticus) for food purposesPublication . Cunha, Nair; Falcão Espada Lopes de Andrade, Vanda Maria; Macedo, Antónia; Lúcia da Mata Silvério Ruivo, Paula; Basto de Lima, Maria GabrielaGlobal population is projected to reach 9.1 billion by 2050, emphasizing the need for increased food production. Edible insects, such as house crickets (Acheta domesticus), emerged as promising due to higher nutritional value and efficient feed conversion rates compared to conventional protein sources. Incorporating insect powders into new food products can improve consumer acceptance but often leads to poor technological food processing functionality and/or undesirable organoleptic characteristics. Protein isolates have proven to be effective in enhancing this functionality and consumer acceptance, but existent protein extraction methods still lack improvements concerning the optimization of protein extraction rates. This study aimed to address this gap by developing and comparing the yield of three different protein extraction methods using sodium hydroxide, ascorbic acid or alcalase from house crickets (Acheta domesticus) for food applications. Protein extraction was performed on cricket powder with a mean protein content of 46.35 g/100 g, and the results were evaluated. The enzymatic method shows the highest protein extraction rate at 69.91% with a mean protein content of 60.19 g/100 g, while extraction with NaOH or ascorbic acid resulted in rates of 60.44 and 46.34%, respectively. Further studies on technological food processing functionality and sensorial evaluation of products developed with this protein extract are recommended.
- A Fish-Based Tool for the Quality Assessment of Portuguese Large RiversPublication . Faro, António Tovar; Ferreira, Maria Teresa; Oliveira, João ManuelMultimetric indices play a pivotal role in assessing river ecological quality, aligning with the European Water Framework Directive (EU WFD) requirements. However, indices developed specifically for large rivers are uncommon. Our objective was to develop a fish-based tool specifically tailored to assess the ecological quality in Portuguese large rivers. Data were collected from seven sites in each of three Portuguese large rivers (Minho, Guadiana, and Tagus). Each site was classified using an environmental disturbance score, combining different pressure types, such as water chemistry, land use, and hydromorphological alterations. The Fish-based Multimetric Index for Portuguese Large Rivers (F-MMIP-LR) comprises four metrics: % native lithophilic individuals; % alien individuals; % migrant individuals; and % freshwater native individuals, representing compositional, reproductive, and migratory guilds. The index showed good performance in separating least- and most-disturbed sites. Least-disturbed sites were rated ‘high’ or ‘good’ by F-MMIP-LR, contrasting with no such classification for most-disturbed sites, highlighting index robustness. The three rivers presented a wide range of F-MMIP-LR values across the gradient of ‘bad’ to ‘high’, indicating that, on a large spatial extent, the biological condition was substantially altered. The F-MMIP-LR provides vital information for managers and decision-makers, guiding restoration efforts and strengthening conservation initiatives in line with the WFD
- Lactic acid bacteria: a sustainable solution against phytopathogenic agentsPublication . Saragoça, A.; Canha, H.; Varanda, Carla; Materatski, Patrick; Cordeiro, A.I.; Gama, J.Biological control agents (BCAs) are beneficial living organisms used in plant protection to control pathogens sustainably. Lactic acid bacteria (LAB) have gained attention in biopesticides due to their safety as recognized by the Food and Drug Administration. These bacteria possess antifungal properties, demonstrating inhibitory effects through nutrient competition or the production of antimicrobial metabolites. Numerous Lactobacillus species have shown the ability to inhibit pathogenic microorganisms, primarily through acid production. The organic acids secreted by LAB reduce the pH of the medium, creating a hostile environment for microorganisms. These organic acids are a primary inhibition mechanism of LAB. This article reviews several studies on LAB as BCAs, focusing on their inhibition modes. Additionally, it discusses the limitations and future challenges of using LAB to control phytopathogens for sustainable agriculture.
- Fluctuations in Mediterranean Diet adherence pre- and post-pandemic : a study of portuguese cohorts 2019–2024Publication . Andrade, Vanda; Pinto, PaulaBackground/Objectives: The Mediterranean Diet (MD) is a lifestyle offering numerous health benefits. Nevertheless, the adherence to the MD is moderate even in Mediterranean countries. While sociodemographic factors influence MD adherence, additional impacts occurred due to the COVID-19 pandemic. This cross-sectional longitudinal study with three cohorts of Portuguese adults analyzes MD adherence before, during, and after the COVID-19 pandemic, and explores the effect of sociodemographic variables. Methods: Sociodemographic factors, lifestyle habits, and MD adherence were assessed in the years 2019, 2021, and 2024 with an online self-filled questionnaire. MD adherence was measured with the Mediterranean Diet Adherence Screener (MEDAS). Results: MEDAS score increased significantly (p < 0.05) from 2019 to 2021 (6.2 ± 0.7 to 7.7 ± 0.1), followed by a significant (p < 0.05) decrease in 2024 (7.2 ± 0.1) relative to 2019, which was more pronounced in participants with higher income. Accordingly, a trend in healthier
- Local factors have a greater influence on the abundance of alfalfa weevil and its larval parasitoids than landscape complexity in heterogeneous landscapesPublication . Levi‑Mourao, A.; Meseguer, R.; Madeira, Filipe; Martinez‑Casasnovas, J.A.; Costamagna, A.C.; Pons, X.Context The alfalfa weevil Hypera postica Gyllenhal (Coleoptera: Curculionidae) is one of the most destructive pests of alfalfa worldwide. Both local and landscape-scale factors can significantly infuence crop pests, natural enemies, and the efectiveness of biological control services, but the relative infuence of these factors is unclear. Objectives We investigated the infuence of the local variables and surrounding landscape composition and configuration on the abundance of alfalfa weevil, and on the abundance and parasitism rates of its larval parasitoids, Bathyplectes spp. Methods We sampled 65 commercial alfalfa fields along the Ebro Basin, Spain, over a period of 3 years, recording the field characteristics and landscape structure at three buffer radii of 250, 500 and 1000 m from the center of each field. Results The abundance of weevil larvae was positively associated with the field perimeter and with the uncut alfalfa surrounding the pipes of the sprinkler irrigation system, but only one configuration variable was positively correlated: the alfalfa edge density. No local characteristics or landscape structures were associated with the abundance of adult weevils. The abundance of Bathyplectes spp. adults was positively associated to local factors such as the densities of alfalfa weevils and aphids. Few landscape structure variables, such as alfalfa edge density and Simpson’s Diversity Index, had explanatory value only at 250 m buffer radius. The rate of larval parasitism was affected by local variables, such as alfalfa weevil abundance and field age. Conclusion Our results provide, for the first time in the Mediterranean region and Europe, evidence of the relative importance of landscape structure and local factors on the abundance of the alfalfa weevil and its larval parasitoids, Bathyplectes spp. The strongest infuences were based on local characteristics.