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Expression of Lupinus albus PR-10 proteins during root and leaf development

authorProfile.affiliationInstituto Politécnico de Santarém, Escola Superior Agrária de Santarémpt_PT
dc.contributor.authorPinto, Paula
dc.contributor.authorRibeiro, A.
dc.contributor.authorRegalado, A. P.
dc.contributor.authorRodrigues-Pousada, C.
dc.contributor.authorRicardo, C. P. P.
dc.date.accessioned2011-06-03T08:39:46Z
dc.date.available2011-06-03T08:39:46Z
dc.date.issued2005
dc.description.abstractBased on the NH2-terminal sequence of three PR-10 isoforms previously identified in Lupinus albus leaves and a conserved amino-acid region in the PR-10 proteins from leguminosae, a pair of oligonucleotides was designed and used to amplify the corresponding cDNA fragment from a L. albus leaves cDNA library. A fragment of DNA of 200 bp was isolated from the polymerase chain reaction (PCR) mixture and subsequently used to screen the cDNA library. A cDNA coding for a PR-10 protein of 158 amino acid residues was cloned and sequenced. Subsequent studies involving Northern and Western blot analysis have shown that the PR-10 protein isoforms are differentially expressed during the development of the healthy lupin plant. High mRNA and protein contents were detected in roots and hypocotyls of both 7- and 20-d-old plants. In young leaves, the mRNA and protein contents were low and increasead in mature leaves. Tissue printing experiments with root sections suggest that the proteins are extracellular and are mainly associated with the vascular tissues in mature roots.por
dc.identifier.citationPINTO, M.P.1; RIBEIRO, A. ; REGALADO; A. P. ; RODRIGUES-POUSADA, C.; RICARDO C. P. (2005) - Expression of Lupinus albus PR-10 proteins during root and leaf development. Biologia Plantarum, 49 (2): 187-193. DOI: 10.1007/s10535-005-7193-2por
dc.identifier.issn0006-3134
dc.identifier.urihttp://hdl.handle.net/10400.15/452
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherSpringerpor
dc.relation.publisherversionhttp://www.springerlink.com/content/uv638041092r44x5/fulltext.pdfpor
dc.subjectcDNA cloningpor
dc.subjectDifferential protein expressionpor
dc.subjectIntercellular spacespor
dc.subjectVascular tissuespor
dc.subjectWhite lupinpor
dc.subjectLupinus albuspor
dc.titleExpression of Lupinus albus PR-10 proteins during root and leaf developmentpor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage193por
oaire.citation.startPage187por
oaire.citation.titleBiologia Plantarumpor
person.familyNamePaula S F S Pinto
person.givenNameMaria
person.identifier.ciencia-id011F-62A8-AE04
rcaap.rightsopenAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication2260faef-53cb-403f-a0ea-637298da3dc7
relation.isAuthorOfPublication.latestForDiscovery2260faef-53cb-403f-a0ea-637298da3dc7

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