Molecular and hydraulic responses of grapevine to water status and  phenology under long-term differential irrigation treatments

Damásio, M.; Pinto, C.; Salguero, J.; Alarcón, M. V.; Deus, J.; David, T. S.; Silvestre, J.; Carvalho, L. C.; Zarrouk, Olfa

Publication

Molecular and hydraulic responses of grapevine to water status and phenology under long-term differential irrigation treatments

2025-09Research article

datacite.subject.fos	Ciências Agrárias
dc.contributor.author	Damásio, M.
dc.contributor.author	Pinto, C.
dc.contributor.author	Salguero, J.
dc.contributor.author	Alarcón, M. V.
dc.contributor.author	Deus, J.
dc.contributor.author	David, T. S.
dc.contributor.author	Silvestre, J.
dc.contributor.author	Carvalho, L. C.
dc.contributor.author	Zarrouk, Olfa
dc.date.accessioned	2025-08-27T16:19:20Z
dc.date.available	2025-08-27T16:19:20Z
dc.date.issued	2025-09
dc.description.abstract	Understanding seasonal interactions among hydraulic, chemical and molecular signalling under water deficit is crucial for improving vineyard irrigation strategies under climate change and increasing water scarcity. We aimed to test how irrigation strategies and phenology affect the hydraulic adjustment mechanisms of eight fieldgrown grapevine varieties under different long-term irrigation regimes: Full-irrigated (100 % ETc), deficitirrigation (50 % Full-irrigated), and non-irrigated. Leaf pre-dawn water potential, leaf hydraulic conductivity (Kleaf), stomatal conductance, aquaporin gene expression and hormone content, were assessed at four key phenological stages, from pea-size to post-harvest. Stomatal closure was the earliest water-stress response across genotypes, while hormonal balance differentiated responsive from non-responsive genotypes regarding chemical signalling. Kleaf was primarily driven by phenology, peaking at early stages and declining thereafter. Aquaporin gene expression was both genotype- and phenology-dependent, with high activity during early stages followed by progressive down-regulation, aligned with changes in stomatal conductance and Kleaf and reflecting each variety iso-/anisohydric behaviour. This pattern confirms the role of aquaporins in grapevine hydraulic adjustments and stomatal regulation under decreasing water potentials. Aquaporins and Kleaf showed positive responses to water availability at post-harvest, indicating differential watering benefits among genotypes. Our results emphasize the need for field-phenotyping studies to fine-tune water management strategies in viticulture.	eng
dc.description.sponsorship	FCT, FEADER
dc.identifier.citation	Damásio, M. ; Pinto, C. ; Salguero; Alarcón, MV ; Deus, J. [1] , [2] ; David, TS; Silvestre, J ; Carvalho, LC ; Zarrouk, O. (2025).Molecular and hydraulic responses of grapevine to water status and phenology under long-term differential irrigation treatments. Agricultural water management, 318. 109708
dc.identifier.doi	https://doi.org/10.1016/j.agwat.2025.109708
dc.identifier.issn	0378-3774
dc.identifier.uri	http://hdl.handle.net/10400.15/5868
dc.language.iso	eng
dc.peerreviewed	yes
dc.publisher	Elsevier
dc.relation	Project COMPETE2030-FEDER-00630300, LISBOA2030-FEDER-00630300 (projet no 14616)-AI4OptiAgri
dc.relation	Project UID/AGR/04129/2019
dc.relation	project UIDP/04129/2020
dc.relation	project UID/00239
dc.relation	project DL57/2016/CP1382/CT0024 to L.C.C
dc.relation.hasversion	https://www.sciencedirect.com/science/article/pii/S0378377425004226?via%3Dihub
dc.rights.uri	http://creativecommons.org/licenses/by-nc/4.0/
dc.subject	Vitis vinifera L.
dc.subject	abscisic acid
dc.subject	aquaporins
dc.subject	auxin
dc.subject	deficit irrigation
dc.subject	drought
dc.subject	stomatal conductance
dc.title	Molecular and hydraulic responses of grapevine to water status and phenology under long-term differential irrigation treatments	eng
dc.type	research article
dspace.entity.type	Publication
oaire.citation.issue	109708
oaire.citation.title	Agricultural water management
oaire.citation.volume	318
oaire.version	http://purl.org/coar/version/c_970fb48d4fbd8a85