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Estudo de extractos de Genista tenera actividade antioxidante e inibição de enzimas do metabolismo da glucose
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Advisor(s)
Abstract(s)
Objectivo: Estudar a actividade antidiabética e antioxidante de extractos de
Genista tenera.
Materiais e métodos: Investigou-se a actividade antioxidante pelo método
das espécies reactivas ao ácido tiobarbitúrico e o método do MTT (3-(4,5-
dimetiltiazol-2-il)-2,5-difenil brometo de tetrazolio). Procurou-se clarificar o
mecanismo de acção antidiabética pelo estudo da actividade inibitória nas
enzimas α-glucosidase, glucose-6-fosfatase e glicogénio fosforilase.
Resultados: No ensaio de MTT os extractos em éter, butanol e acetato de
etilo possuem boa actividade antioxidante (87,80 %, 67,82 % e 67,70 % de
viabilidade celular respectivamente). Na α-glucosidase os extractos em butanol e
acetato de etilo apresentaram inibição (0,97% e 2,36% de actividade enzimática).
Os extractos em acetato de etilo, butanol e éter são inibidores da glucose-6-
fosfatase (48,33%, 80,25% e 64,42% de actividade enzimática).
Conclusões: Os extractos de Genista tenera em acetato de etilo, butanol e
éter poderão ser no futuro incluídos em nutracêuticos para prevenir ou tratar a
diabetes tipo 2.
Objectives: To study the antidiabetic and antioxidant activity of Genista tenera extracts. Materials and methods: The antioxidant activity was investigated using the thiobarbituric acid assay and the MTT (3-(4,5-dimethiltiazol-2-il)-2,5-diphenyl tetrazol bromide) method. The mechanism of antidiabetic activity was evaluated in terms of inhibitory action on the enzymes α-glucosidase, glucose-6-phosphatase and glycogen phosphorylase. Results: The MTT assay showed a good antioxidant activity for the ether, butanol and ethyl acetate extracts. (87,80 %, 67,82 % e 67,70 % of cellular viability respectively). For α-glucosidase the butanol and ethyl acetate extracts showed strong inibitory activity (0,97% e 2,36% of enzymatic activity). The ethyl acetate, buthanol and ether extracts are moderate inhibitors of glucose-6- phosphatase (48,33%, 80,25% e 64,42% of enzymatic activity). Conclusions: Genista tenera extracts in ethyl acetate, buthanol and ether can be included in nutraceutical products to prevent and treat type 2 diabetes in the future.
Objectives: To study the antidiabetic and antioxidant activity of Genista tenera extracts. Materials and methods: The antioxidant activity was investigated using the thiobarbituric acid assay and the MTT (3-(4,5-dimethiltiazol-2-il)-2,5-diphenyl tetrazol bromide) method. The mechanism of antidiabetic activity was evaluated in terms of inhibitory action on the enzymes α-glucosidase, glucose-6-phosphatase and glycogen phosphorylase. Results: The MTT assay showed a good antioxidant activity for the ether, butanol and ethyl acetate extracts. (87,80 %, 67,82 % e 67,70 % of cellular viability respectively). For α-glucosidase the butanol and ethyl acetate extracts showed strong inibitory activity (0,97% e 2,36% of enzymatic activity). The ethyl acetate, buthanol and ether extracts are moderate inhibitors of glucose-6- phosphatase (48,33%, 80,25% e 64,42% of enzymatic activity). Conclusions: Genista tenera extracts in ethyl acetate, buthanol and ether can be included in nutraceutical products to prevent and treat type 2 diabetes in the future.
Description
Keywords
Genista tenera Flavonoíde Diabetes Antioxidante Glucosidase Nutracêutico Alimento funcional Flavonoides Antioxidant α-glucosidase Nutraceutic Functional food
Citation
Santos, I. M. M. T. (2011). Estudo de extractos de Genista tenera actividade antioxidante e inibição de enzimas do metabolismo da glucose. 124p. Dissertação de mestrado em Sistemas de Prevenção e Controlo Alimentar, Escola Superior Agrária de Santarém, Instituto Politécnico de Santarém. Disponível em:http://hdl.handle.net/10400.15/1282