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Bioactivity studies and chemical profile of the antidiabetic plant Genista tenera

2009Journal article Open access
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authorProfile.affiliationInstituto Politécnico de Santarém, Escola Superior Agrária de Santarémpt_PT
dc.contributor.authorRauter, A. P.
dc.contributor.authorMartins, F.
dc.contributor.authorLopes, R.
dc.contributor.authorFerreira, J.
dc.contributor.authorSerralheiro, L. M
dc.contributor.authorAraújo, M. E.
dc.contributor.authorBorges, C
dc.contributor.authorJustino, Jorge
dc.contributor.authorV.M. Silva, Filipa
dc.contributor.authorGoulart, Margarida
dc.contributor.authorThomas-Oates, J.
dc.contributor.authorRodriges, J. A. P
dc.contributor.authorEdwards, E
dc.contributor.authorNoronha, J. P.
dc.contributor.authorPinto, R.
dc.contributor.authorMota-Filipe, H.
dc.date.accessioned2011-08-08T09:20:15Z
dc.date.available2011-08-08T09:20:15Z
dc.date.issued2009
dc.description.abstractAim of the study: Genista tenera is a plant endemic to the island of Madeira and is used in folk medicine to control diabetes. In the present work we evaluate the antihyperglycaemic activity of its n-butanol extract and determine its chromatographic profile. In addition, this extract, the ethyl acetate and diethyl ether plant extracts were studied in order to assess the plant antioxidant and acetylcholinesterase inhibitory activities, as well as its cyto- and genotoxicities. Materials and methods: HPLC-DAD-ESI-MS was used to analyze the flavonoid profile of the n-butanol extract. The antihyperglycaemic activity of this extract was performed over streptozotocin induced diabetic Wistar rats (200mg/kg, bw/day), for 15 days. Antioxidant activity (DPPH assay) and acetylcholinesterase inhibitory effect (Ellman method) were also performed. Acute cytotoxicity and genotoxicity were assessed by proliferative index quantification and the short-term chromosomal aberration technique, after exposure of lymphocytes to the extracts. Results and conclusions: The n-butanol extract, where 21 monoglycosyl and 12 diglycosyl flavonoids were detected, significantly lowered blood glucose levels, bringing them to normal values after 15 days of treatment. The best radical scavenging activity was observed for the ethyl acetate extract (48.7% at 139.1μg/mL), which was also the most effective one at the minimal concentration tested. The highest acetylcholinesterase inhibitory activity (77.0% at 70. 0μg/mL) was also obtained with the ethyl acetate extract. In vitro toxicity studies showed no evidence for acute cytotoxicity or genotoxicity. This is the first report on antidiabetic activity of genus Genista.por
dc.description.sponsorshipMCES, FCT, British Council; BBSRC and Merck, Sharp & Dohmepor
dc.identifier.citationRauter, A. P. ; Martins, F; Lopes, R.; Ferreira, J. ; Serralheiro, L. M. ; Araújo, M. E. ; Borges, C. ; Justino, J.1 ; Silva, F. V.1 ; Goulart, M.1; Thomas-Oates, J. ; Rodriges, J. A. P.; Edwards, E.; Noronha, J. P. ; Pinto, R. Mota-Filipe, H. (2009) - Bioactivity studies and chemical profile of the antidiabetic plant Genista tenera. Journal of ethnopharmacology, 122 (2): 384-393. Retrieved from: http://www.sciencedirect.com/science/article/pii/S0378874108005588. doi: 10.1016/j.jep.2008.10.011por
dc.identifier.issn0378-8741
dc.identifier.urihttp://hdl.handle.net/10400.15/486
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.relationProject EU (ICA4-CT-2001-10056)por
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S0378874108005588por
dc.subjectGenista tenerapor
dc.subjectLeguminosaepor
dc.subjectAntihyperglycaemic activitypor
dc.subjectAntioxidant activitypor
dc.subjectAcetylcholinesterase inhibitionpor
dc.subjectToxicitypor
dc.subjectHPLC-DAD-ESI-MSpor
dc.subjectEtnobotânicapor
dc.subjectComposto fenólicopor
dc.subjectAntioxidantepor
dc.subjectDiabetespor
dc.subjectIlha da Nadeirapor
dc.titleBioactivity studies and chemical profile of the antidiabetic plant Genista tenerapor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage393por
oaire.citation.startPage384por
oaire.citation.titleJournal of ethnopharmacologypor
person.familyNameJustino
person.familyNameVinagre Marques da Silva
person.givenNameJorge
person.givenNameFilipa
person.identifierhttps://scholar.google.pt/citations?hl=pt-PT&user=TcUz9ZAAAAAJ
person.identifier.ciencia-idB610-66DA-A3AC
person.identifier.orcid0000-0003-3114-5926
person.identifier.orcid0000-0003-4700-2938
person.identifier.scopus-author-id22942414000
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication3e483773-36b8-4a7b-82f3-c0a39f6a7fa9
relation.isAuthorOfPublication344bf736-31c9-4a35-844c-67a176174a44
relation.isAuthorOfPublication.latestForDiscovery344bf736-31c9-4a35-844c-67a176174a44

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