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New In Vitro studies on the bioprofile of Genista tenera antihyperglycemic extract

2015Journal article Open access
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dc.contributor.authorBatista, D.
dc.contributor.authorFalé, P.L.
dc.contributor.authorSerralheiro, M.L.
dc.contributor.authorAraújo, M.E.
dc.contributor.authorMadeira, P.J.A.
dc.contributor.authorBorges, C.
dc.contributor.authorTorgal, Isabel
dc.contributor.authorGoulart, Margarida
dc.contributor.authorJustino, Jorge
dc.contributor.authorMartins, A.
dc.contributor.authorRauter, A.P.
dc.date.accessioned2023-03-20T11:37:20Z
dc.date.available2023-03-20T11:37:20Z
dc.date.issued2015
dc.description.abstractThe inhibition of a-glucosidase and glucose-6-phosphatase, two enzymes involved in the carbohydrate metabolism, is an important target to control glycaemia on individuals with type 2 diabetes. In this work we report for the first time the inhibition of both enzymes by the antihyperglycemic n-butanol extract from Genista tenera (Fabaceae). This extract decreased a-glucosidase and glucose-6-phosphatase activities to 0.97 and 80.25 %, respectively, being more effective than acarbose, and phlorizin, the positive controls, which reduced enzymes activities only to 17.39 and 96.06 %. Once inflammation and oxidative stress are related to diabetic impairments, the anti-inflammatory activity of the extract was also evaluated, through its inhibitory activity over COX-1 enzyme (47.5 % inhibition). Moreover, after induction of oxidative stress by UV radiation, the viability of irradiated rat liver hepatoma cells exposed to the extract was significantly higher (67.82 %) than that promoted by ascorbic acid, the positive control (45.05 %). In addition, the stability of the extract under gastrointestinal conditions was evaluated by HPLC–DAD-ESI–MS/MS. Flavonoid diglycosides were identified as the main constituents of the extract, and no alterations in the chemical composition nor in the antioxidant activity were observed after in vitro digestion with artificial gastric and pancreatic juices.pt_PT
dc.description.sponsorshipFundação para a Ciência e a Tecnologia e Comissão Europeiapt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationBatista, D.; Falé, P.L.; Serralheiro, M.L.; Araújo, M.E.; Madeira, P.J.A.; Borges, C.; Torgal, I.; Goulart, M.; Justino, J.; Martins, A. & Rauter, A.P. (2015). New In Vitro studies on the bioprofile of Genista tenera antihyperglycemic extract. Natural Products and Bioprospecting, 5, 277–285. https://doi.org/10.1007/s13659-015-0077-zpt_PT
dc.identifier.doihttps://doi.org/10.1007/s13659-015-0077-zpt_PT
dc.identifier.urihttp://hdl.handle.net/10400.15/4361
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringerpt_PT
dc.relationPTDC/QUI/67165/2006 - FCTpt_PT
dc.relationPEst-OE/QUI/UI0612/2013 - FCTpt_PT
dc.relationProject "PERsonalised ICT supported Service for Independent Living and Active Ageing" - FP7-ICT-2013-10, Project Nr. 610359, 2013-2016 - European Commissionpt_PT
dc.relation.publisherversionhttps://link.springer.com/article/10.1007/s13659-015-0077-zpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjecta-Glucosidase inhibitionpt_PT
dc.subjectInibição da a-Glucosidasept_PT
dc.subjectIn vitro digestionpt_PT
dc.subjectDigestão In vitropt_PT
dc.subjectHPLC–DAD-ESI–MS/MSpt_PT
dc.subjectAntioxidant activitypt_PT
dc.subjectActividade antioxidantept_PT
dc.subjectFlavonoid glycosidespt_PT
dc.subjectGlicosídeos flavonóidespt_PT
dc.titleNew In Vitro studies on the bioprofile of Genista tenera antihyperglycemic extractpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage285pt_PT
oaire.citation.startPage277pt_PT
oaire.citation.titleNatural Products and Bioprospectingpt_PT
oaire.citation.volume5pt_PT
person.familyNameTorgal
person.familyNameJustino
person.givenNameIsabel
person.givenNameJorge
person.identifier.orcid0000-0001-5774-1318
person.identifier.orcid0000-0003-3114-5926
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication97374359-94ba-4524-8aae-4e97d3d6b294
relation.isAuthorOfPublication3e483773-36b8-4a7b-82f3-c0a39f6a7fa9
relation.isAuthorOfPublication.latestForDiscovery3e483773-36b8-4a7b-82f3-c0a39f6a7fa9

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