Browsing by Author "Lopes, R."
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- Bioactivity studies and chemical profile of the antidiabetic plant Genista teneraPublication . Rauter, A. P.; Martins, F.; Lopes, R.; Ferreira, J.; Serralheiro, L. M; Araújo, M. E.; Borges, C; Justino, Jorge; V.M. Silva, Filipa; Goulart, Margarida; Thomas-Oates, J.; Rodriges, J. A. P; Edwards, E; Noronha, J. P.; Pinto, R.; Mota-Filipe, H.Aim of the study: Genista tenera is a plant endemic to the island of Madeira and is used in folk medicine to control diabetes. In the present work we evaluate the antihyperglycaemic activity of its n-butanol extract and determine its chromatographic profile. In addition, this extract, the ethyl acetate and diethyl ether plant extracts were studied in order to assess the plant antioxidant and acetylcholinesterase inhibitory activities, as well as its cyto- and genotoxicities. Materials and methods: HPLC-DAD-ESI-MS was used to analyze the flavonoid profile of the n-butanol extract. The antihyperglycaemic activity of this extract was performed over streptozotocin induced diabetic Wistar rats (200mg/kg, bw/day), for 15 days. Antioxidant activity (DPPH assay) and acetylcholinesterase inhibitory effect (Ellman method) were also performed. Acute cytotoxicity and genotoxicity were assessed by proliferative index quantification and the short-term chromosomal aberration technique, after exposure of lymphocytes to the extracts. Results and conclusions: The n-butanol extract, where 21 monoglycosyl and 12 diglycosyl flavonoids were detected, significantly lowered blood glucose levels, bringing them to normal values after 15 days of treatment. The best radical scavenging activity was observed for the ethyl acetate extract (48.7% at 139.1μg/mL), which was also the most effective one at the minimal concentration tested. The highest acetylcholinesterase inhibitory activity (77.0% at 70. 0μg/mL) was also obtained with the ethyl acetate extract. In vitro toxicity studies showed no evidence for acute cytotoxicity or genotoxicity. This is the first report on antidiabetic activity of genus Genista.
- Participatory selection of indicators for water resources planning and strategic environmental assessment in PortugalPublication . Santos Coelho, Rosa; Lopes, R.; Coelho, Pedro; Ramos, T.B.; Antunes, P.International agencies, non-governmental organisations, technical experts, and academia have strongly recommended that water resources planning and strategic environmental assessment (SEA) processes should be supported by indicators that can be used to characterise, assess and monitor water resources overtime against management objectives. Moreover, indicators are used to measure the performance of actions implemented to goals and provide simple and meaningful information to stakeholders. The main goal of this research was the operationalization of a stakeholder engagement process for the selection of key indicators to support water resources planning and SEA processes. Those indicators can be adapted to all Portuguese river basin districts and be used through consecutive cycles of planning. The process was developed in four main stages: (1) collection of suitable indicators; (2) analysis and screening of indicators, (3) stakeholders' workshop and (4) reflection and refinement of the indicator set. A set of 35 key indicators were selected as the most relevant by stakeholders in the participatory workshop. A final analysis carried out by the research team resulted in a streamlined set with 22 indicators organised in four thematic areas (resources, socioeconomics, governance and risks). This set of key indicators may not be able to capture all specific planning objectives for all river basin regions. In such cases, it will be necessary to add specific indicators to the set proposed herein. This research demonstrated the relevance and usefulness of using a participatory approach in developing a set of key indicators for supporting water resources planning and SEA processes, and the proposed methodology can be used in different contexts. The participatory process fostered stakeholders' commitment to the planning and SEA processes and assured increased fitness of the selected indicators.