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Identification of candidate genes involved in olive response to anthracnose for a sustainable disease management

2023-05Conference object Open access
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authorProfile.affiliationInstituto Politécnico de Santarém_Escola Superior Agráriapt_PT
dc.contributor.authorInácio, Diogo
dc.contributor.authorFélix, M.R.
dc.contributor.authorCampos, Catarina
dc.contributor.authorPatanita, Mariana
dc.contributor.authorRibeiro, Joana A.
dc.contributor.authorVaranda, Carla
dc.contributor.authorMateratski, Patrick
dc.contributor.authorAlbuquerque, André
dc.contributor.authorOsa, Nicolás
dc.contributor.authorPeixe, A.
dc.contributor.authorCampos, Maria Doroteia
dc.date.accessioned2024-01-10T11:23:41Z
dc.date.available2024-01-10T11:23:41Z
dc.date.issued2023-05
dc.description.abstractAnthracnose is a disease that affect the olive tree caused by fungi of the genus Colletotrichum. These fungi are responsible to premature fruit drop and a consequent decrease in the oil quality, and also cause defoliation of trees compromising the production of the following years. Currently the best control strategy is based on application of synthetic fungicides, with a regulatory pressure in agriculture worldwide to limit its use. Looking for a sustainable disease management, the present study reports the transcriptional changes of olive genes that encode enzymes directly associated with the maintenance of the balance of oxidative oxygen species (ROS), in response to Colletotrichum sp. infection. The cultivar selected for the studies was ‘Galega vulgar’, known to be extremely susceptible to this disease. Plants used in the experiments were from in vitro culture (to warranty their healthy status), transplanted to pots and maintained under controlled conditions. Leaves of the olive plants were inoculated with a spore suspension of Colletotrichum sp., and leaf samples were collected before fungi inoculation (T0) and at 10 days (T1) and 35 days (T2) after inoculation. Confirmation of the presence of the fungi in inoculated plants was performed following a real-time qPCR approach. The selected target genes for expression analysis were Superoxide dismutase (SOD), Endochitinase_EP3-like (CHI2), Glutathione S-transferase L3-like (TransFL3), Glutathione peroxidase 2 (PEROX2), Glutathione S-transferase THETA 1 (THETA), Glutathione S-transferase DHAR2-like (TransfDHAR), Glutathione peroxidase 5 (PEROX5) and Glutathione peroxidase 8 (PEROX8). Following a qPCR approach, a general up-regulation of the target genes was detected, but only CHI2 revealed a significantly up-regulation (p<0.005) between T0 and T2 in response to Colletotrichum sp. infection, being a promising candidate to be later used in functional analysis. We emphasize the importance of this study for the identification of candidate genes to incorporate new sources of resistance of olive trees to anthracnose with the promotion of the development of sustainable management strategies.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationInácio, D.; Félix, M.d.R.; Campos, C.; Patanita, M.; Ribeiro, J.A.; Varanda, C.M.R.; Materatski, P.; Albuquerque, A.; Garrido, N.; Peixe, A.; Campos, M.D. (2023). Identification of Candidate Genes Involved in Olive Response to Anthracnose for a Sustainable Disease Management. 1º International meeting, Applied Agro-Biotechnology, 30-31 May, Beja, Portugal. Book of abstracts, p.20pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.15/4682
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherCEBAL - Centro de Biotecnologia Agrícola e Agro-Alimentar do Alentejopt_PT
dc.relationUIDB/05183/2020pt_PT
dc.relation.publisherversionhttps://aabim.cebal.pt/wp-content/uploads/2023/05/AABIM2023_BoA.pdfpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectolive productionpt_PT
dc.subjectsustainabilitypt_PT
dc.subjectanthracnosept_PT
dc.subjectdisease resistancept_PT
dc.subjectgene expression analysispt_PT
dc.titleIdentification of candidate genes involved in olive response to anthracnose for a sustainable disease managementpt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.citation.conferencePlaceCebal, Bejapt_PT
oaire.citation.startPage20pt_PT
oaire.citation.titleBook of abstracts: New approaches for a qualitative valorization of the olive oil sectorpt_PT
person.familyNameFernandes Félix
person.familyNameMurteira Rico dos Santos Campos
person.familyNameCardoso Patanita
person.familyNameAmaro Ribeiro
person.familyNameVaranda
person.familyNameMateratski
person.familyNameAlbuquerque
person.familyNameGarrido de la Osa
person.familyNameVieira Peixe
person.familyNameMurteira Rico da Costa Campos
person.givenNameMaria do Rosário
person.givenNameMaria Catarina
person.givenNameMariana
person.givenNameJoana
person.givenNameCarla
person.givenNamePatrick
person.givenNameAndré
person.givenNameNicolás
person.givenNameAugusto António
person.givenNameMaria Doroteia
person.identifierL-1118-2016
person.identifierO-7518-2018
person.identifierhttps://www.researchgate.net/profile/Mariana_Patanita
person.identifierhttps://www.researchgate.net/profile/Joana-Ribeiro-55
person.identifierw-2502-2018
person.identifierhttps://scholar.google.com/citations?hl=pt-PT&user=02CJdCQAAAAJ
person.identifierhttps://www.researchgate.net/profile/Augusto_Peixe/scores
person.identifierY-5626-2018
person.identifier.ciencia-id821D-579B-959C
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person.identifier.scopus-author-id8899391700
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rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
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