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qPCR as a sensitive tool for detecting Fusarium spp. in tomato plants

2023-06Conference object Open access
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authorProfile.affiliationInstituto Politécnico de Santarém_Escola Superior Agráriapt_PT
dc.contributor.authorCampos, Maria Doroteia
dc.contributor.authorVaranda, Carla
dc.contributor.authorPatanita, Mariana
dc.contributor.authorRibeiro, Joana A.
dc.contributor.authorCampos, Catarina
dc.contributor.authorMateratski, Patrick
dc.contributor.authorAlbuquerque, André
dc.contributor.authorOsa, Nicolás
dc.contributor.authorFélix, M.R.
dc.date.accessioned2024-01-10T11:52:55Z
dc.date.available2024-01-10T11:52:55Z
dc.date.issued2023-06
dc.descriptionApresentação em painelpt_PT
dc.description.abstractThe need to increase food production together with the demand to reduce the application of synthetic chemicals that have consequences on increasing the carbon footprint and negative impacts on the environment and human health, led to the search for alternative methods to protect plants against pathogens. The use of the highly sensitive real-time quantitative PCR (qPCR) arises as an extremely useful tool for studying various agents of infection in plants, such as fungi, viruses, or bacteria leading to a better control of diseases and limiting the use of chemical defence strategies. Given the high incidence of diseases in tomato plants caused by Fusarium spp., their consequent negative economic impacts, and the fact that most phytosanitary treatments are based on the application of synthetic fungicides, the establishment of a molecular-based tool that enables their early and accurate detection is of great interest. Furthermore, it will provide an additional tool for the screening of resistant plants. In the presented study, a TaqMan®-based qPCR method targeting the Fusarium spp.- specific internal transcribed spacer (ITS) region was developed for the simultaneous detection and quantification of a panoply of Fusarium species that affect tomato. As a proof of principle, the new qPCR assay was used to assess Fusarium spp. contamination of tomato field plants and of plants grown under controlled conditions. qPCR combined with the chemistry of TaqMan® MGB probes represents a highly specific and sensitive detection system, even when low amounts of target DNA are present, as in the case of early plant–fungi interactions.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationCampos, M.D.; Varanda, C.M.R.; Patanita, M.; Ribeiro, J.A.; Campos, C.; Materatski, P.; Albuquerque, A.; Garrido, N.; Félix, M.d.R. (2023). qPCR as a sensitive tool for detecting Fusarium spp. in tomato plants. Science CHANGing Policy, La Change Meeting. 2nd June, Évora, Portugal.Book of abstracts, p.100.pt_PT
dc.identifier.isbn978-972-778-331-1
dc.identifier.urihttp://hdl.handle.net/10400.15/4684
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherUniversidade de Évorapt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.titleqPCR as a sensitive tool for detecting Fusarium spp. in tomato plantspt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.citation.conferencePlaceÉvorapt_PT
oaire.citation.startPage100pt_PT
oaire.citation.titleBook of abstracts: Science Changing Policypt_PT
person.familyNameMurteira Rico da Costa Campos
person.familyNameVaranda
person.familyNameCardoso Patanita
person.familyNameAmaro Ribeiro
person.familyNameMurteira Rico dos Santos Campos
person.familyNameMateratski
person.familyNameAlbuquerque
person.familyNameGarrido de la Osa
person.familyNameFernandes Félix
person.givenNameMaria Doroteia
person.givenNameCarla
person.givenNameMariana
person.givenNameJoana
person.givenNameMaria Catarina
person.givenNamePatrick
person.givenNameAndré
person.givenNameNicolás
person.givenNameMaria do Rosário
person.identifierY-5626-2018
person.identifierw-2502-2018
person.identifierhttps://www.researchgate.net/profile/Mariana_Patanita
person.identifierhttps://www.researchgate.net/profile/Joana-Ribeiro-55
person.identifierO-7518-2018
person.identifierhttps://scholar.google.com/citations?hl=pt-PT&user=02CJdCQAAAAJ
person.identifierL-1118-2016
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rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
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