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Publication
Desenvolvimento de bioformulações para o controlo de doenças fúngicas na cultura do morango
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Authors
Abstract(s)
Este projeto teve início em dezembro de 2022 e terminou em novembro de 2023. Todo
o trabalho foi realizado nas instalações fabris da Asfertglobal, que se situam em Perofilho,
Santarém.
Este projeto teve como objetivo realizar bioformulações de origem vegetal e bacteriana
com o intuito de avaliar o seu efeito no controlo das doenças Fusariose e Podridão Cinzenta na
cultura do morango.
Após a sua formulação, estes produtos foram sujeitos a testes de estabilidade e
envelhecimento, sendo selecionados os que obtiveram melhores resultados para testes de
antagonismo in vitro contra os fungos Fusarium incarnatum e Botrytis cinerea. Após serem
realizados os testes, as formulações foram testadas nas plantas para avaliar a sua eficácia contra
os fungos mencionados.
Os resultados demonstraram que as formulações de origem vegetal e bacterianos tinham
potencial para inibir os fungos Fusarium incarnatum e Botrytis cinerea, contudo, quando
aplicadas nos morangueiros infetados, embora tenham tido um impacto positivo no controlo
das doenças, não alcançaram a mesma eficácia dos produtos comerciais de referência.
A formulação bacteriana Whole Broth (células bacterianas e metabolitos excretados) foi a
que mais se destacou quando aplicada aos morangueiros, mostrando resultados semelhantes aos
produtos comerciais de referência em termos de frequência e severidade das doenças, bem
como na massa seca das plantas infetadas, no entanto, todas as formulações necessitam de ser
reformuladas para serem consideradas alternativas viáveis aos produtos comerciais no controlo
destas doenças.
This project started in December 2022 and concluded in November 2023. All work was carried out at the Asfertglobal facilities located in Perofilho, Santarém. The objective of this project was to develop plant and bacterial-based bioformulations to assess their effectiveness in controlling Fusarium wilt and gray mold diseases in strawberry cultivation. After formulation, these products underwent stability and aging tests, with the bestperforming ones selected for in vitro antagonism tests against the fungi Fusarium incarnatum and Botrytis cinerea. Following these tests, the formulations were evaluated on plants to assess their efficacy against the mentioned fungi. The results demonstrated that both plant-based and bacterial formulations had potential to inhibit Fusarium incarnatum and Botrytis cinerea fungi. Despite the positive effect of the formulations on disease control, they did not achieve the same level of efficacy as the commercial reference products when applied to infected plants. The bacterial formulation Whole Broth (bacterial cells and excreted metabolites) stood out the most when applied to strawberries, showing results comparable to commercial reference products in terms of disease frequency and severity, as well as the dry weight of infected plants. Nonetheless, all formulations need to be reformulated to be considered viable alternatives to commercial products for controlling these diseases.
This project started in December 2022 and concluded in November 2023. All work was carried out at the Asfertglobal facilities located in Perofilho, Santarém. The objective of this project was to develop plant and bacterial-based bioformulations to assess their effectiveness in controlling Fusarium wilt and gray mold diseases in strawberry cultivation. After formulation, these products underwent stability and aging tests, with the bestperforming ones selected for in vitro antagonism tests against the fungi Fusarium incarnatum and Botrytis cinerea. Following these tests, the formulations were evaluated on plants to assess their efficacy against the mentioned fungi. The results demonstrated that both plant-based and bacterial formulations had potential to inhibit Fusarium incarnatum and Botrytis cinerea fungi. Despite the positive effect of the formulations on disease control, they did not achieve the same level of efficacy as the commercial reference products when applied to infected plants. The bacterial formulation Whole Broth (bacterial cells and excreted metabolites) stood out the most when applied to strawberries, showing results comparable to commercial reference products in terms of disease frequency and severity, as well as the dry weight of infected plants. Nonetheless, all formulations need to be reformulated to be considered viable alternatives to commercial products for controlling these diseases.
Description
Dissertação, Mestrado, Engenharia Agronómica, Instituto Politécnico de Santarém, Escola Superior Agrária, 2024
Keywords
Fragaria morango Fusarium incarnatum Botrytis cinerea controlo de doenças das plantas óleo essencial essential oils strawberries plant disease control experimentação experimentation
Citation
Borbinha, L. S. C. (2024). Desenvolvimento de bioformulações para o controlo de doenças fúngicas na cultura do morango. Dissertação de Mestrado na área da Engenharia Agronómica, apresentada na Escola Superior Agrária de Santarém.