Browsing by Author "Garrido, Nuno Domingos"
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- Association between ACE D allele and elite short distance swimmingPublication . Costa, Aldo Matos; Silva, António José; Garrido, Nuno Domingos; Louro, Hugo; de Oliveira, Ricardo Jacó; Breitenfeld, LuizaThe influence of ACE gene on athletic performance has been widely explored, and most of the published data refers to an I/D polymorphism leading to the presence (I allele) or absence (D allele) of a 287-bp sequence in intron 16, determining ACE activity in serum and tissues. A higher I allele frequency has been reported among elite endurance athletes, while the D allele was more frequent among those engaged in more power-orientated sports. However, on competitive swimming, the reproducibility of such associations is controversial. We thus compared the ACE genotype of elite swimmers with that of non-elite swimming cohort and of healthy control subjects. We thus sought an association of the ACE genotype of elite swimmers with their competitive distance. 39 Portuguese Olympic swimming candidates were classified as: short (<200 m) and middle (400-1,500 m) distance swimmers, respectively. A group of 32 non-elite swimmers were studied and classified as well, and a control group (n = 100) was selected from the Portuguese population. Chelex 100 was used for DNA extraction and genotype was determined by PCR-RFLP methods. We found that ACE genotype distribution and allelic frequency differs significantly by event distance only among elite swimmers (P < or = 0.05). Moreover, the allelic frequency of the elite short distance swimmers differed significantly from that of the controls (P = 0.021). No associations were found between middle distance swimmers and controls. Our results seem to support an association between the D allele and elite short distance swimming.
- Effects of Backpack Load and Trekking Poles on Energy Expenditure During Field Track WalkingPublication . Brito, João; Garrido, Nuno Domingos; Hopffer Romero, Félix Luís; de Araújo Junior, Adenilson; Reis, VictorThis study evaluates the effects of the use of backpack load and trekking poles on feld track walking energy expenditure. Twenty male volunteer pole walkers (age: 22.70 ± 2.89 years; body mass: 77.90 ± 11.19 kg; height: 1.77 ± 0.06 m; percentage of body fat: 14.6 ± 6.0 %) walked at a self-selected pace on a pedestrian feld track over a period of more than six months. Each subject was examined at random based on four walking conditions: non-poles and non-load, with poles and non-load, nonpoles and with load, with poles and with load. Heart rate, oxygen uptake and energy expenditure were continuously recorded by a portable telemetric system. Non-load walking speed was lower during walking with poles when compared with no poles (p ≤ 0.05). Oxygen uptake, energy expenditure and heart rate varied signifcantly across different conditions. Our results suggest that the use of trekking poles does not influence energy expenditure when walking without an additional load, but it can have an effect during backpack load walking. Moreover, our results indicate that the use of trekking poles may not be helpful to lower the exertion perceived by the subjects when walking with an additional load.