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Browsing by Author "Elias, Miguel"

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  • Autochthonous Starter Cultures Are Able to Reduce Biogenic Amines in a Traditional Portuguese Smoked Fermented Sausage
    Publication . Dias, Igor; Laranjo, Marta; Potes, Maria Eduarda; Agulheiro-Santos, Ana Cristina; Ricardo-Rodrigues, Sara; Fialho, Ana Rita; Véstia, Joana; Fraqueza, Maria João; Oliveira, Margarida; Elias, Miguel
    Traditional smoked fermented sausages are highly appreciated in Portugal and are mostly manufactured according to traditional procedures. The aim of the present work was to evaluate the effect of autochthonous starter cultures on the safety and quality of a smoked fermented sausage, Painho da Beira Baixa (PBB), preserving its sensory quality. Physicochemical parameters, namely pH and water activity (aW), microbiological parameters, biogenic amines, colour, texture profile and sensory attributes were assessed. Different starters were selected based on our previous work. Staphylococcus equorum S2M7, Staphylococcus xylosus CECT7057, Lactobacillus sakei CV3C2, Lactobacillus sakei CECT7056 and a yeast strain (2RB4) were co-inoculated in meat batters at defined concentrations. Starters had a significant effect on the reduction of pH. Enterobacteria and Listeria monocytogenes were not detected in inoculated end-product sausages. Moreover, sausages inoculated with S. equorum S2M7/L. sakei CV3C2/yeast 2RB4 showed a significant reduction in the total content of biogenic amines. No significant differences between treatments were observed for colour and texture parameters, except for adhesiveness. The studied starters did not compromise the sensory characteristics of PBB. To our knowledge, this is the first comprehensive study on the quality and safety of this type of smoked fermented sausage from the central region of Portugal.
    2020Journal article Open access Show more
  • Co-inoculation with Staphylococcus equorum and Lactobacillus sakei reduces vasoactive biogenic amines in traditional dry-cured sausages
    Publication . Dias, Igor; Laranjo, M.; Potes, M.E.; Agulheiro Santos, A.C.; Ricardo-Rodrigues, S.; Fialho, A.R.; Fraqueza, M.J.; Oliveira, Margarida; Elias, Miguel
    Dry-cured sausages are traditional in Mediterranean countries, and Paio do Alentejo (PA) is one of the most popular in South Portugal. The aim of the present work was to evaluate the effect of combined starters on the safety and quality of PA preserving its sensory quality. Physicochemical parameters, namely pH and water activity (aW), microbiological parameters, biogenic amines, color, texture, and sensory attributes were assessed. Three starter cultures were used, namely Staphylococcus equorum S2M7 and Lactobacillus sakei CV3C2, both separate and combined with the 2RB4 yeast strain at a concentration of 106 cfu/g. Dextrose 0.25% was added to the meat batter. Starters had a significant effect on the reduction of aW values (0.845 to 0.823). The treatment with L. sakei as well as the co-inoculation of L. sakei with S. equorum effectively reduced the L. monocytogenes counts to undetectable levels. Sausages co-inoculated with S. equorum S2M7/L. sakei CV3C2 showed a significant reduction in the content of vasoactive amines, namely tryptamine (26.21 to 15.70) and β-phenylethylamine (4.80 to 3.69). Regarding texture, control PA showed higher hardness values, and the starters promoted the cohesiveness of the batter while reducing chewiness. The studied starters did not compromise the sensory characteristics of PA
    2021Journal article Open access Show more
  • Fiambre de peixe: preferência da cor e aceitabilidade
    Publication . Ribeiro, Ana Teresa; Elias, Miguel; Teixeira, Bárbara; Mendes, Rogério
    Foram preparados fiambres de corvina para se estudar a preferência do consumidor relativamente ao tipo de fiambre e, em particular, à sua cor. As cores dos fiambres foram manipuladas pela adição individual e em diferentes proporções de pigmento de cochonilha (tons vermelhos), Fibricolor (tons rosa) e Instanka sugar powder 75/700 (tons castanhos). Fotografias das amostras serviram de base para a criação de um inquérito “on line”. Neste inquérito, além de se procurar perceber o perfil do inquirido e dos seus hábitos alimentares, solicitou-se a escolha das cores expectáveis ou mais atraentes para um fiambre de peixe. Do total de 2237 inquiridos, cerca de 78 % apresentaram idades compreendidas entre os 44 anos ou menos, evidenciando-se nestes o bacharelato ou licenciatura como grau de formação. Quanto ao consumo de fiambre, o género feminino evidenciou preferência pelo fiambre de peru e o género masculino pelo fiambre de porco. A frequência de consumo de fiambre e de peixe foi semelhante, “3x por semana”. Mais de metade dos inquiridos (55%) respondeu “não sei” à possível compra de fiambre de peixe. O fiambre de corvina preparado com 0,0005 % de cochonilha, cuja cor foi muito semelhante à do fiambre de corvina sem corante, teve uma boa aceitabilidade por parte dos inquiridos
    2021Journal article Open access Show more