Liquid chromatography–diode array detection–electrospray ionisation mass spectrometry/nuclear magnetic resonance analyses of the anti-hyperglycemic flavonoid extract of Genista tenera Structure elucidation of a flavonoid-C-glycoside

The anti-hyperglycemic flavonoid extract obtained from Genista tenera was first studied by liquid chromatography (LC)–diode array detection (DAD) which showed the presence of two major compounds. One of them was identified as genistein-7-O-glucoside. Luteolin-7-O-glucoside was detected as a minor constituent, while luteolin-7,3′-di-O-glucoside and rutin were found in trace amounts. LC–DAD–ESI–MS and NMR were used to confirm the structure of these compounds and allowed the elucidation of the structure of the unknown major compound, which is the flavonoid 5,7,4′-trihydroxyisoflavone-8-C-glucoside.

Keywords

Genista tenera LC–DAD–ESI–MS NMR flavonoid glycoside 5,7,4′-Trihydroxyisoflavone-8-C-glucoside

URI

http://hdl.handle.net/10400.15/4536

Citation

Rauter, A.P.; Martins, A.; Borges, C.; Ferreira, J.; Justino, J.; Bronze, M.R.; Coelho, A.V.; Choi, Y.H. & Verpoorte, R. (2005). Liquid chromatography–diode array detection–electrospray ionisation mass spectrometry/nuclear magnetic resonance analyses of the anti-hyperglycemic flavonoid extract of Genista tenera Structure elucidation of a flavonoid-C-glycoside. Journal of Chromatography A, 1089(1-2), 59-64. DOI:10.1016/j.chroma.2005.06.046