Justino, JorgeRauter, AméliaDias, CatarinaMartins, AliceBranco, IsabelGoulart, MargaridaNogueira, JoséNeng, NunoSilva, FilipaTrevitt, ClareWaltho, Jon2013-12-092013-12-092012-06Justino,J.;Rauter,A.;Dias,C.;Martins.A.;Branco,I.;Goulart,M. (2012).Non-toxic Salvia sclareoides Brot. extracts as a source of functional food ingredients: Phenolic profile, antioxidant activity and prion binding properties.Food Chemistry,132,1930-1935. Disponível em : http://www.sciencedirect.com/science/article/pii/S03088146110178330308-8146http://hdl.handle.net/10400.15/882Estudo de produção agronómica de espécis da salvia com vista à avaliação dos seus constituintes para o potencial controlo da doença de AlzaimerSalvia sclareoides is an aromatic herb native to Portugal, of which phenolic content (Folin–Ciocalteau method), chemical profile (HPLC/DAD), antioxidant activity (DPPH, β-carotene/linoleic acid assays), acute toxicity (MTT method, adapted for non-adherent cells), genotoxicity (short-term chromosomal aberration assay) and prion binding properties were evaluated in the acetone, water, ethanol, methanol and n-butanol extracts. The latter presented the highest phenolic content and antioxidant activity (DPPH assay), and was the single one with the flavonoids (+)-catechin, kaempferol O-glucoside and quercetin. Vanillic acid was the major component of all extracts but gallic, gentisic, caffeic, syringic, coumaric and ferulic acids were also found in some extracts. Only the n-butanol extract had components binding to the cellular form of human prion protein detected by NMR which showed specificity for two regions of the folded domain and for the unstructured N-terminal region. Extracts were not cytotoxic nor genotoxic, reinforcing the potential of S. sclareoides for nutraceutical purposes.porSalvia sclareoidesPhenolic profileAntioxidant activityCytotoxicityGenotoxicityPrion bindingjournal article